Plasminogen Activator Inhibitor: DISCUSSION(6)

This hypothesis is based on the fact that these two events occur at stages VII-VIII of the cycle in the rat, at which PA secretion is at its greatest. Since peritubular cells produce PAI-1 basally and PAI-1 cannot cross the blood-testis barrier, PAI-1 of peritubular cell origin probably counteracts Sertoli cell PA secreted basally. By…

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Plasminogen Activator Inhibitor: DISCUSSION(5)

Determining whether the in vitro data presented here reflect a potential physiological role of PAI-1 in the male gonad function requires further studies. Undoubtedly, upon culturing on plastic, cells adapt to the in vitro conditions. Precedents exist showing that specific parameters like transferrin expression for Sertoli cells or PAI-1 expression for endothelial cells gradually increase…

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Plasminogen Activator Inhibitor: DISCUSSION(4)

Since Sertoli cells can apparently synthesize the two PAs and PAI-1, it is important to determine the nature of the signal(s) directing them to produce PA or PAI-1. One candidate may be the tandem TGFp1 and FSH. TGFp1 is known to favor the synthesis of matrix components and of protease inhibitors, and to decrease FSH-in-duced…

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Plasminogen Activator Inhibitor: DISCUSSION(3)

However, they differed from those reported previously on the hormonal regulation of PAI-1 in monkey and mouse Sertoli cells. Indeed, it was found that FSH stimulated, whereas cAMP analogues inhibited, PAI-1. This discrepancy remains unclear. One explanation may be that the authors of the two papers used a less purified FSH (FSH-s17, FSH activity 20XNIH-FSH-S1)…

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Plasminogen Activator Inhibitor: DISCUSSION(2)

One explanation may be that peritubular cells, which actively proliferate in vitro (in contrast to Sertoli cells), lose their differentiated status and transform into fibroblasts with time in culture. This will be the subject of further investigation. canadian health&care mall

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Plasminogen Activator Inhibitor: DISCUSSION(1)

The aim of the present study was to investigate whether Sertoli cells could express PAI-1 and what its regulation was. This was achieved by using a model of cultured Sertoli cells recovered from 20-day-old rats, and zymographic, Northern blotting, and Western blotting procedures. proventil inhaler

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Plasminogen Activator Inhibitor: RESULTS(4)

We also performed Western blotting experiments 1) to determine whether PAI-1 expressed by Sertoli cells migrated at 49 and 46 kDa as previously described with purified rat PAI-1, and 2) to determine the effects of bFGF and of TGFp1 at the protein level. By Western blotting, two proteins that migrated at 49 and 46 kDa…

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