Production of Gelatinases and Tissue Inhibitors: DISCUSSION(6)

Phorbol ester PMA has been used to examine regulatory mechanisms in many studies of proteolytic enzymes, although it is a nonphysiological factor. It has been shown that PMA can induce the production of gelatinase B through a 12-0-tetradecanoylphorbol-13-acetate-responsive element in the promoter region of the gelatinase B gene and can activate gelatinase A through protein…

Read more

Production of Gelatinases and Tissue Inhibitors: DISCUSSION(5)

TGFp is thought to be an intraovarian factor involved in follicle development. Studies demonstrate that TGFp is present in human ovarian stromal, granulosa, and theca cells, and porcine theca cells during follicle growth. Evidence shows that TGFp may act on the target cells in an autocrine/paracrine manner. On the other hand, TGFp is also involved…

Read more

Production of Gelatinases and Tissue Inhibitors: DISCUSSION(4)

Two proteins with inhibitory activity on gelatinases were found in EOSC-conditioned medium and had approximate molecular masses of 29 and 22 kDa, similar to mouse TIMP-1 and TIMP-2, respectively. A third inhibitor of 24 kDa was found exclusively in ECM and also had the same molecular mass as mouse TIMP-3. All these proteins were heat-…

Read more

Production of Gelatinases and Tissue Inhibitors: DISCUSSION(3)

EOSC secreted enzymes of approximately 72 and 92 kDa into conditioned medium, and these enzymes possessed gelatinolytic activity. Conditioned medium of EOSC treated with APMA, a substance used in vitro to activate latent forms of gelatinases, contained two extra enzymes of approximately 68 and 82 kDa. The gelatinolytic activity normally observed in conditioned medium from…

Read more

Production of Gelatinases and Tissue Inhibitors: DISCUSSION(2)

Myofibroblasts are typically found in connective tissues undergoing remodeling. In addition to their contraction function, myofibroblasts have been found to take part in tissue remodeling by producing several proteolytic enzymes, such as urokinase-type plasminogen activator and type-1 plasminogen activator inhibitor by myofibroblasts of human breast cancer, and TIMPs by human skin fibroblasts. Thus, myofibroblasts in…

Read more

Production of Gelatinases and Tissue Inhibitors: DISCUSSION(1)

The results of this study demonstrate that cells obtained from stromal tissue at the apex of developing follicles in the equine ovary are fibroblast-like in shape and express type I procollagen and a-SMA. Bovine ovary stromal cells assume a fibroblast-like morphology in vitro. Other¬†authors have reported a-SMA-positive cells in the stroma of human ovaries. While…

Read more

Production of Gelatinases and Tissue Inhibitors: RESULTS(5)

Effects of Different Doses of TGF/3 or PMA on TIMP Production by EOSC The effects of increasing concentrations of TGFp (1-10 ng/ml) or PMA (5-20 ng/ml) on TIMP-1 and TIMP-2 activity in conditioned medium were assessed by reverse gelatin zymography. EOSC were treated with TGFp or PMA for 48 h. A representative reverse gelatin zymogram…

Read more