Expression of Monocyte Chemoattractant Protein: DISCUSSION(2)

There must also be a signal that causes increased expression of mRNA encoding MCP-1 within luteal tissue. The expression of mRNA encoding MCP-1 was significantly increased in CL from animals after functional luteo-lysis and also from one animal prior to the completion of functional luteolysis (progesterone > 1 ng/ml, cow 959, Table 1). The potentially…

Read more

Expression of Monocyte Chemoattractant Protein: DISCUSSION(1)

The results of this study demonstrate that MCP-1 mRNA is present within the bovine CL between Days 15 and 20 of the estrous cycle. Most significantly there is an increased amount of MCP-1 mRNA in CL after functional luteolysis compared to CL in the days leading up to luteolysis. High mRNA expression was seen in…

Read more

Expression of Monocyte Chemoattractant Protein: RESULTS(3)

In the preluteolytic animals the most intense signal was seen in a CL collected from a cow (number 959) that, out of the three preluteolytic animals studied, had the highest plasma PGFM concentration and lowest progesterone concentration at the time of tissue collection (Table 1). Buy Advair Diskus Online Immunohistochemistry Immunohistochemistry was performed on serial…

Read more

Expression of Monocyte Chemoattractant Protein: RESULTS(2)

RT-PCR A 270-base pair (bp) cDNA product was amplified by RT-PCR. The PCR products from 8 animals (3 preluteolytic and 5 postluteolytic) are shown in Figure 1a. The identity of the band was confirmed by DNA sequencing and was 100% homologous with the expected cDNA sequence described by Wempe et al.. The intensity of the…

Read more

Expression of Monocyte Chemoattractant Protein: RESULTS(1)

Progesterone Analysis Thirteen animals had systemic progesterone concentrations consistent with a functional CL (systemic progesterone concentration > 1 ng/ml) at the time of slaughter. Plasma progesterone concentration (mean ± SEM) was 3.6 ± 1.0 ng/ml (range 1.9-6.9 ng/ml). Five animals had undergone luteolysis at the time of slaughter (systemic progesterone concentration < 1 ng/ml). The…

Read more

Expression of Monocyte Chemoattractant Protein: MATERIALS AND METHODS(5)

Image Analysis The intensity of the in situ hybridization signal was analyzed using an NIH-Image analysis system (NIH, Bethes-da, MD). The number of graphic pixels occupied by silver grains (identified by a set gray threshold) within a defined area of the tissue section was counted and presented as a percentage of the total pixel number…

Read more

Expression of Monocyte Chemoattractant Protein: MATERIALS AND METHODS(4)

MCP-1 Riboprobes The MCP-1 cDNA amplified by the PCR reaction described in the previous section was cloned into pGEM-T (Promega) using the manufacturer’s procedure. The insert was sequenced to determine its identity and orientation within the plasmid. The construct was linearized with NcoI and used as a template to generate 35S-labeled antisense RNA using SP6…

Read more