Expression of the Inhibitor of Т-Cell Apoptosis (ita) Gene: DISCUSSION(8)

This speculation is supported by the observations (discussed above) that 1) both bcl-Xiong and ita mRNA levels are significantly increased within granulosa cells from follicles most recently selected into the preovulatory hierarchy (e.g., 9- to 12-mm follicles) as compared to pooled granulosa cell layers from all follicles 6-8 mm in diameter ; Fig. 1) and…

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Expression of the Inhibitor of Т-Cell Apoptosis (ita) Gene: DISCUSSION(7)

Although it has been proposed that the expression of both Bcl-2 and Bcl-xiong proteins provides a protective effect against the initiation of apoptosis by forming dimers with the death-promoting Bcl-2-related protein Bax in a variety of tissues, we have recently determined that constitutive expression of bcl-xhoag mRNA within hen granulosa cells is considerably greater than…

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Expression of the Inhibitor of Т-Cell Apoptosis (ita) Gene: DISCUSSION(5)

Similar to what is seen in prehierarchal follicles, ita mRNA levels in preovulatory follicle granulosa cells increase after 24 h of culture, although the extent of increase is not as dramatic. While it remains possible that the relatively smaller change in static mRNA levels is due to both an increase in ita mRNA transcription and…

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Expression of the Inhibitor of Т-Cell Apoptosis (ita) Gene: DISCUSSION(4)

The observation of increased ita mRNA during atresia is similar to that previously reported for urokinase plasminogen activator ) and bcl-2 mRNA, but is in direct contrast to the marked decrease observed for several other mRNA transcripts that have been evaluated (e.g., FSH receptor , LH receptor mRNA , bcl-xLon/, , ich-1 ]. While increased…

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Expression of the Inhibitor of Т-Cell Apoptosis (ita) Gene: DISCUSSION(3)

Moreover, the spontaneous increase in ita mRNA is evident within 2 h of suspension culture (Fig. 5) and occurs prior to the time when significant oligonucleosome formation (a hallmark of apoptosis) can first be detected . Induced expression of ita mRNA in granulosa cells following suspension culture is consistent with results from incubated spleen cells…

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Expression of the Inhibitor of Т-Cell Apoptosis (ita) Gene: DISCUSSION(2)

The ita gene is also expressed in the theca layer, and similar to what is seen with Ьс1-хи,п„, there is a lack of obvious change in mRNA levels with regard to follicle development. Significantly greater levels of ita mRNA were detected within the stroma layer of follicles, but given that ovarian thecal and stromal tissues…

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