Loss of the Signature Six Carboxyl Amino Acid Tail: DISCUSSION(3)

Extensive differences in individual animal responses to IFN-т treatment occurred in the present study, irrespective of the dose of ovIFN-т administered (Fig. 2). Others have also observed such disparate responses to ovIFN-т treatment. It is not clear why such variable responses occur, but it may well be that failure of individual animals to respond robustly…

Read more

Loss of the Signature Six Carboxyl Amino Acid Tail: DISCUSSION(2)

It was interesting to note that p3, as well as its truncated counterpart, were at least 200-fold more potent in antiproliferative activity than were ovine and bovine IFN-ts tested previously. Their activities approached those of huIFN-a2 and huIFN-w in the same assay, presumably because ovIFN-т-рЗ possesses a much higher affinity for the human type I…

Read more

Loss of the Signature Six Carboxyl Amino Acid Tail: DISCUSSION(1)

Because IFN-ts and their homologues, the IFN-cos, have six amino acid extensions at their carboxyl termini, it has been tempting to speculate that this structural feature might contribute to the function of these IFNs. It could, for example, allow them either to recruit additional polypeptide components to the receptor complex or to interact with the…

Read more

Loss of the Signature Six Carboxyl Amino Acid Tail: RESULTS(3)

There were no detectable differences in the responses to p3 and рЗТшб when administered at 300 (xg/day. Both proteins were able to extend CL life span when administered at 60 (xg/day. The mean duration of CL life span for those ewes receiving 60 |xg/day of рЗТшб was numerically lower but was not different statistically from…

Read more

Loss of the Signature Six Carboxyl Amino Acid Tail: RESULTS(2)

The antiviral potencies of p3 and рЗТтб were within the range of activities determined for other ovine and bovine IFN-ts and for human (hu) IFN-a2 in this and other laboratories. buy asthma inhalers The concentration of рЗТтб required to inhibit the proliferation of the human lymphoma cell line, Daudi, by 50% relative to controls was…

Read more

Loss of the Signature Six Carboxyl Amino Acid Tail: RESULTS(1)

Recombinant ovlFN-т Production From 50 to 100 mg of enriched p3 or рЗТтб ovIFN-t were generated per liter of fermentation medium. The apparent molecular mass of p3 (19-20 kDa) was similar to that of the ovIFN-ts produced by ovine conceptuses. As expected, the рЗТгпб protein, which lacked the hexapeptide sequence (GDLNSP) at its carboxyl terminus,…

Read more

Loss of the Signature Six Carboxyl Amino Acid Tail: MATERIALS AND METHODS(5)

Both probes were synthesized through PCR by using KlenTaq and P.F.U. polymerases, vector-specific primers, 100 jJuCi [a-32P]dATP, 20 |xM dATP, and 200 |xM each of deoxycytidine triphosphate (dCTP), deoxyguanidine triphosphate (dGTP), and deoxythymidine triphosphate (dTTP). Both hybridizations were performed at 42°C for 16 h, and the membrane was washed and exposed to XAR film at…

Read more