MATERIALS AND METHODS(4)

Statistical Analysis

Testis volumes were not standardized according to body weight, as we believe that absolute testis size is a better indicator of reproductive maturation than relative testis size. A number of previous studies have shown body weight to be a poor predictor of sperm count, and photoperiod can affect body weight independently of testis size in rodents (e.g., ). Our previous work on F344 rats showed similar qualitative results using adjusted or unadjusted measurements of testis size. In experiment 2, in particular, we were interested in the effect of FR on absolute measures of reproductive development, not on reproductive development relative to somatic growth, and so adjustment by body weight was inappropriate. levitra super active plus

Data on food intake were analyzed by repeated measures ANOVA. In experiments 1 and 2, data were analyzed by repeated measures two-way ANOVA for each experiment, followed by two-way ANOVA for each week with post-hoc contrast comparisons of all pairs of means. Attained significance levels < 0.05 were considered significant. Analyses were performed using Superanova (v 1.11) or Statview+ Graphics (v 1.04 A; both by Abacus Concepts, Berkeley, CA) on a Power Macintosh 6100 computer (Apple Computer, Cupertino, CA). All means are presented with their standard errors.

Category: Androgen / Tags: Androgen, Neonatal, Rats, Reproductive Maturation