The level of FR (30%) was chosen after a pilot experiment that showed a very slight inhibition of testicular growth in LD by 30% food reduction, and little or no effect of 20% or 10% food reduction. Rats held in SD voluntarily reduced their food intake by approximately 10-15% from Week 3 until the preliminary experiment was terminated at 10 wk (p < 0.05; Fig. 1). Thus, the FR treatments were 30% of the amount of food eaten by same-age ad lib-fed controls in the same photoperiod. FR rats were fed daily between 1400 and 1700 h. Testes and body weight were measured at 2-wk intervals, beginning after 1 wk of photoperiod and FR treatment, according to the procedures of experiment 1. As in experiment 1, all measurements presented were taken by a single person (RWD) blind with respect to treatment. ventolin inhaler
Originally all treatments had 10 subjects. Two animals in the LD-FR group developed testicular abnormalities in one or both testes. All three of these testes became extremely flaccid and cyanotic (as seen through the skin of the scrotum), and on autopsy at Week 9 appeared dark blue-gray and had an unusually prominent vasculature with possible varicosities. Both animals were removed from the study. Finally, one rat in the SD-FR group died under anesthesia. Thus, final cell sizes were 8-10 rats/treatment group.