Macrophages are involved in the rapid destruction of luteal tissue that occurs after functional luteolysis. MCP-1 has been described in porcine luteal cells, and increased expression is seen in the CL of the rat prior to the influx of monocytes/macrophages in late-stage luteal tissue and following prolactin-induced regression, findings that support the hypothesis of MCP-1 involvement in luteolysis. buy ventolin inhalers
As in other species, macrophages are involved in destruction of bovine luteal tissue after luteolysis, and a role for MCP-1 in controlling macrophage influx in the bovine CL has been suggested. In addition, we have shown previously that there is a significant increase in the number of T lymphocytes (specifically CD8 +, cytotoxic/ suppressor T cells) within the bovine CL from Day 16 of the estrous cycle onward, prior to luteolysis. The reason for this influx of T lymphocytes is unclear, but T lymphocytes are a potential source of MCP-1. In this study our primary aim was to investigate the expression of MCP-1 mRNA within the bovine CL during luteolysis using both the reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization. In addition, in order to assess whether T lymphocytes might be involved in MCP-1 mRNA expression in the bovine CL, we used immunohis-tochemistry to analyze the distribution of these cells within sections of luteal tissue and correlated this with the distribution of MCP-1 mRNA.