The value is similar to the rate of 1- and 2-cell porcine embryos matured and fertilized in vivo. Taken together with these results, our findings indicate that the higher incidence of fragmentation and degeneration of embryos was caused by polyspermic fertilization. Therefore, if the incidence of polyspermy is suppressed, the effect of hyaluronic acid on embryo could be confirmed. buy antibiotics online
In conclusion, the present results indicated that hyaluronic acid supports the development of porcine oocytes matured and fertilized in vitro to the blastocyst stage and that the effect is maximal at a concentration of 0.5 mg/ml. Similar results were obtained with embryos cultured in chon-droitin sulfate А-supplemented medium at concentrations of 0.1 and 0.5 mg/ml. Further experiments must be conducted to determine the mechanism by which hyaluronic acid and chondroitin sulfate A support embryonic development, although addition of hyaluronic acid to the simple culture medium described here provides an effective way to culture porcine embryos derived from follicular oocytes matured and fertilized in vitro to blastocysts.