Testicular Germ Cell Transplantation: Conclusion 2

It is evident that the proportions of donor-origin spermatozoa in recipient semen fluctuate at different time points (Fig. 4). It is impossible to detect the proportion of donor-origin spermatozoa in recipient ejaculate before AI to maximize the chance of producing donor-derived offspring because of the lengthy time required for microsatellite detection of semen and preparation…

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Testicular Germ Cell Transplantation: Conclusion

The outcome of colonization is also dependent upon the optimal time of transfer of the donor cells. The timing of transplants must take into account when the greatest numbers of stem cell niches are available after endogenous stem cell removal, as well as the health of the testicular environment to support donor cell colonization. Although…

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Testicular Germ Cell Transplantation: DISCUSSION

This study investigated the feasibility of testis germ cell transplantation between the same and different breeds of sheep, as well as the functionality of donor sperm produced in recipient testis. Using microsatellite markers, our study demonstrated that testis germ cell transplantation between unrelated immunocompetent rams of different breeds is feasible and that the resultant recipients…

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Testicular Germ Cell Transplantation: RESULTS 2

As shown in Figure 2, the mean SCs of control animals increased consistently over time from 24 to 30 cm during 16 wk. On average, the testis SCs increased 1 cm every 18.6 days. The size of the testes decreased rapidly following irradiation in both treatment groups 2-4 wk after irradiation. The SCs did not…

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Testicular Germ Cell Transplantation: RESULTS

In the pilot experiment (Table 3), two animals at the peripubertal stage were irradiated with a single dose of 9 or 15 Gy. The left testes of two recipient rams were injected with donor cells labeled with fluorescent dye PHK26 16 wk after irradiation and were removed by castration after 2 wk to evaluate the…

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Testicular Germ Cell Transplantation: Lambing and Paternity Test

Synchronization of Ewes and AI with Recipient Semen Merino ewes were kept on pasture with water and hay available ad libitum. Estrus was synchronized in animals using sponges impregnated with 30 mg of flugestone acetate (Ovagest; Bioniche), which were inserted into the vagina for 15 days. At sponge removal, animals received an i.m. injection of…

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Testicular Germ Cell Transplantation: Detection of Donor Cells

Detection of Donor Cells in Recipient Testis and Donor DNA in Recipient Ejaculate In the first experiment, the left testes of two recipients at the prepubertal stage were removed by castration 2 wk after transplantation to evaluate the location of the transferred cells, while the right testes were kept in place for long-term assessment of…

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