Elevation of Inducible Nitric Oxide Synthase: DISCUSSION(4)

An increasing body of evidence suggests that endometrial ap-optosis may be orchestrated by cytokines, which are mainly produced by stromal lymphoid cells that provide the appropriate microenvironment for adjacent epithelial cells. In particular, tumor necrosis factor alpha and interleukin-1 were shown to be increasingly secreted during the late-secretory phase immediately before menstruation approaches and thus might…

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Elevation of Inducible Nitric Oxide Synthase: DISCUSSION(3)

Moreover, lack of eNOS staining in glandular epithelium when frozen sections were used does not necessarily exclude moderate epithelial eNOS expression, as shown using paraffin sections, in which additional antigen moieties could have been unmasked by microwave treatment. Thus, epithelial eNOS may play a part in the inhibition of platelet aggregation within the endometrium, as…

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Elevation of Inducible Nitric Oxide Synthase: DISCUSSION(2)

This may reflect the fact that iNOS mRNA and protein expression precede its enzyme activity. Our results on increased iNOS enzyme activity during menstruation are consistent with those obtained at the mRNA level by Tseng et al.. However, increased iNOS message could be observed by the latter authors only when isolated endometrial glands of menstruating…

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Elevation of Inducible Nitric Oxide Synthase: DISCUSSION(1)

NO is a potent agonist of apoptosis. However, whether its production might be involved in the regulation of menstrual shedding, a process associated with extensive ap-optosis, has not been elucidated until now. The results of the present study show that endometrial iNOS activity was low during the proliferative and late-secretory phase and increased significantly until…

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Elevation of Inducible Nitric Oxide Synthase: RESULTS(2)

However, in several cases, the menstru-al-phase endometria displayed a slightly increased staining intensity in the disintegrating functionalis layer compared to the basal layer, although these moderate differences were not significant (data not shown). buy cheap antibiotics eNOS immunoreactivity was restricted to vascular endothelial cells (Fig. 3C), whereas nNOS staining was not detectable (data not shown)….

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Elevation of Inducible Nitric Oxide Synthase: RESULTS(1)

A summary of the results is given in Table 1. NOS Activity The calcium-independent NOS activity in menstrual-phase endometria was about six times that of proliferative or late-secretory endometria. In contrast, the calcium-dependent fraction of the enzyme activity remained unchanged during the phases of the menstrual cycle investigated. birth control yasmin Demonstration of NOS mRNA…

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Elevation of Inducible Nitric Oxide Synthase: MATERIALS AND METHODS(6)

After extensive rinsing in TRIS-buffered saline, the sections were treated with alkaline phosphatase-labeled anti-digoxigenin antibody (1:500 dilution) for 1 h at room temperature. The color reaction was developed with x-phosphatase. Negative controls were performed by omission of terminal transferase. Sections were counterstained with haemalaun. All enzymes and reagents were purchased from Boehringer (Mannheim, Germany). buy…

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