A new antiarrhythmic principle (part 2)


We therefore investigated whether it might also be possible to enhance cellular coupling. The first substance of this type was AAP10, for which an antiarrhythmic effect has already been shown in an isolated rabbit heart model subjected to ischemia reperfusion injury . We have attempted to clarify further the mechanism of action and to determine the quaternary structure of AAP10.

Heart preparation and epicardial mapping: All experiments were performed in accordance with the ethical rules of the Council for International Organization of Medical Science and German laws for animal welfare. The method of heart preparation and epicardial potential mapping has been described in more detail previously . Briefly, male white New Zealand rabbits (conventional, normally fed ad libitum, weighing 1500 to 1800 g, obtained from Fortkamp, Lengerich, Germany) were treated with 1000 U/kg heparin intravenously for 5 mins before they were sacrificed by a sharp blow to the neck, with subsequent exsanguination.The heart was excised, prepared and perfused according to the Langendorff technique at constant pressure (70 cm H2O) with Tyrode’s solution of the following composition (in mmol/L): Na+ 161.02, K+ 5.36, Ca2+ 1.8, Mg2+ 1.05, Cl-147.86, HCO3- 23.8, PO42- 0.42 and glucose 11.1, equilibrated with 95% oxygen and 5% carbon dioxide. The surface temperature of the heart was 37°C. The hearts were connected to a 256-channel mapping system (HAL3, temporal resolution 4 kHz/channel; amplitude resolution 0.04 mV, interchannel coupling less than -60 dB; ELSA GmbH, Aachen, Germany) as described previously . Two hundred and fifty-six AgCl electrodes were affixed to four polyester plates (in 8×8 orthogonal matrixes with an interelectrode distance of 1 mm), which were attached smoothly to the heart surface, so that they could easily follow the heart’s movements without dislocation. You will love this opportunity to shop with best pharmacy on the internet and pay less every time you visit: buy ortho tri-cyclen online here to discover exactly how much less you could be spending while still getting your treatment exactly the way you need it.

Category: Cardiology / Tags: Antiarrhythmic drugs, Antiarrhythmic peptides, Cardiac arrhythmia, Cellular coupling, Gap junction

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